Everyone does drug test different and it usually is a matter of what they are trying to accomplish and cost efficiency. I've filled a few cups for USADA. The procedure I explained is how it is done at a very high level. This keeps it from being a question of contamination and you actually get to watch the B sample opened and poured into the testing beaker. If the B sample is positive then you are suspended. You can appeal with USADA or WADA, but you wont win.

If they are doing tires, its not the first time they have done them. I'd bet one of the Mafia member's Coors Light on it.